Author:
Houng H S,Lynn A R,Rosen B P
Abstract
Calcium transport was investigated in membrane vesicles prepared from the oral bacterium Streptococcus sanguis. Procedures were devised for the preparation of membrane vesicles capable of accumulating 45Ca2+. Uptake was ATP dependent and did not require a proton motive force. Calcium transport in these vesicles was compared with 45Ca2+ accumulation in membrane vesicles from Streptococcus faecalis and Escherichia coli. The data support the existence of an ATP-driven calcium pump in S. sanguis similar to that in S. faecalis. This pump, which catalyzes uptake into membrane vesicles, would be responsible for extrusion of calcium from intact cells.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference10 articles.
1. Calcium efflux from Escherichia coli. Evidence for two systems;Ambudkar S. V.;J. Biol. Chem.,1984
2. The ATP-dependent Ca 2l-pumping system of Streptococcus faecium;Burkler J.;Ann. N. Y. Acad. Sci.,1982
3. Carafoli E. G. Inesi and B. P. Rosen. 1984. Calcium transport across biological membranes p. 129-185. In H. Sigel (ed.) Metal ions in biological systems. Marcel Dekker Inc. New York.
4. Light induced generation of a protonmotive force and Ca 2+- transport in membrane vesicles of Streptococcus cremoris fused with bacteriorhodopsin proteoliposomes;Driessen A. J. M.;Biochim. Biophys. Acta,1985
5. Calculator programs for computing the composition of the solutions containing multiple metals and ligands used for experiments in skinned muscle cells;Fabiato A.;J. Physiol. (Paris),1979
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