Expression of the bacteriophage T4 denV structural gene in Escherichia coli

Author:

Recinos A,Augustine M L,Higgins K M,Lloyd R S

Abstract

The expression of the T4 denV gene, which previously had been cloned in plasmid constructs downstream of the bacteriophage lambda hybrid promoter-operator oLpR, was analyzed under a variety of growth parameters. Expression of the denV gene product, endonuclease V, was confirmed in DNA repair-deficient Escherichia coli (uvrA recA) by Western blot analyses and by enhancements of resistance to UV irradiation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference21 articles.

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4. Partial complementation of the UV sensitivity of E. coli and yeast excision repair mutants by the cloned denV gene of bacteriophage T4;Chenevert J. M.;Mol. Gen. Genet.,1986

5. Review article: DNA repair of ultraviolet-irradiated bacteriophage T4;Friedberg E. C.;Photochem. Photobiol.,1975

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