Author:
Lyerly D M,Sullivan N M,Wilkins T D
Abstract
Antibodies against Clostridium difficile toxin A were purified by affinity chromatography from antiserum prepared against crude C. difficile toxin preparations. The affinity-purified antibody preparation was free of detectable amounts of antibodies to other C. difficile antigens, as demonstrated by crossed immunoelectrophoresis, and specifically neutralized the cytotoxicity of toxin A. An indirect enzyme-linked immunosorbent assay (ELISA) was subsequently developed using the antibody preparation for the specific detection of toxin A. The ELISA, which could detect 1 ng (5 ng/ml) of toxin A, was used to quantitate the toxin in the culture supernatant fluids of strains of C. difficile. The ELISA values for toxin A closely correlated with the toxin A and B cytotoxic titers of the supernatant fluids. In addition, toxin A was detected by ELISA in human fecal specimens from persons with antibiotic-associated colitis, demonstrating that this toxin is produced during C. difficile colitis.
Publisher
American Society for Microbiology
Reference36 articles.
1. Axelsen N. H. J. Kr 0il and B. Weeke (ed.). 1973. A manual of quantitative immunoelectrophoresis. Methods and applications. Universitetsforlaget Oslo Norway.
2. Two toxins (D-1 and D-2) of Clostridium difficile causing antibiotic-associated colitis: purification and some characterization;Banno Y.;Biochem. Int.,1981
3. Antibiotic-associated pseudomembranous colitis;Bartlett J. G.;Rev. Infect. Dis.,1979
4. Laboratory diagnosis of antibioticassociated colitis;Bartlett J. G.;Lab. Med.,1981
5. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia;Bartlett J. G.;N. Engl. J. Med.,1978
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