Affiliation:
1. Plant-Bacteria Interactions Laboratory, Departamento Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Profesor Albareda 1, E-18008 Granada, Spain
Abstract
ABSTRACT
Sinorhizobium meliloti
1021 carries two megaplasmids, pSymA of 1,354 kb and pSymB of 1,683 kb, which are essential in establishing symbiosis with its legume hosts and important for bacterial fitness in the rhizosphere. We have previously shown that pSymA is self-transmissible and that its conjugal functions are regulated by the transcriptional repressor RctA. Here, we show conjugal transfer of pSymB as an in
trans
mobilization event that requires the type IV secretion system encoded by pSymA. pSymB carries a functional
oriT
and an adjacent relaxase gene,
traA2
, that is also transcriptionally repressed by
rctA
. Both symbiotic megaplasmids would require the relaxase genes in
cis
with their respective
oriT
s to achieve the highest transfer efficiencies.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
29 articles.
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