pSymA-Dependent Mobilization of the Sinorhizobium meliloti pSymB Megaplasmid

Abstract

ABSTRACT Sinorhizobium meliloti 1021 carries two megaplasmids, pSymA of 1,354 kb and pSymB of 1,683 kb, which are essential in establishing symbiosis with its legume hosts and important for bacterial fitness in the rhizosphere. We have previously shown that pSymA is self-transmissible and that its conjugal functions are regulated by the transcriptional repressor RctA. Here, we show conjugal transfer of pSymB as an in trans mobilization event that requires the type IV secretion system encoded by pSymA. pSymB carries a functional oriT and an adjacent relaxase gene, traA2 , that is also transcriptionally repressed by rctA . Both symbiotic megaplasmids would require the relaxase genes in cis with their respective oriT s to achieve the highest transfer efficiencies.