Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA-Reference-Cited by-同舟云学术

Magnetic Bead Capture Eliminates PCR Inhibitors in Samples Collected from the Airborne Environment, Permitting Detection of Pneumocystis carinii DNA

Published:2001-01 Issue:1 Volume:67 Page:449-452
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Maher Nancy¹,Dillon H. Kenneth¹,Vermund Sten H.¹,Unnasch Thomas R.¹

Affiliation:

1. Division of Geographic Medicine and Department of Environmental Health Sciences, University of Alabama at Birmingham, Birmingham, Alabama

Abstract

ABSTRACT PCR detection methods are useful in studies of organisms not amenable to culture. Inhibitors in environmental samples can interfere with such assays. We describe a magnetic bead DNA capture protocol that removes inhibitors from outdoor air samples, maintaining the sensitivity of a 16S Pneumocystis carinii mitochondrial rRNA gene-based PCR.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.1.449-452.2001

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