Detection of Indigenous Halobacillus Populations in Damaged Ancient Wall Paintings and Building Materials: Molecular Monitoring and Cultivation-Reference-Cited by-同舟云学术

Detection of Indigenous Halobacillus Populations in Damaged Ancient Wall Paintings and Building Materials: Molecular Monitoring and Cultivation

Published:2001-10 Issue:10 Volume:67 Page:4891-4895
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Piñar Guadalupe¹,Ramos Cayo²,Rölleke Sabine³,Schabereiter-Gurtner Claudia¹,Vybiral Dietmar¹,Lubitz Werner¹,Denner Ewald B. M.¹

Affiliation:

1. Institute of Microbiology and Genetics, University of Vienna, A-1030 Vienna, Austria1;

2. Institute of Microbiology, Technical University of Denmark, Lyngby, Denmark2; and

3. Genalysis GmbH, D-14943 Luckenwalde, Germany3

Abstract

ABSTRACT Several moderately halophilic gram-positive, spore-forming bacteria have been isolated by conventional enrichment cultures from damaged medieval wall paintings and building materials. Enrichment and isolation were monitored by denaturing gradient gel electrophoresis and fluorescent in situ hybridization. 16S ribosomal DNA analysis showed that the bacteria are most closely related to Halobacillus litoralis . DNA-DNA reassociation experiments identified the isolates as a population of hitherto unknown Halobacillus species.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.10.4891-4895.2001

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