Genetic and Molecular Organization of the Alkylbenzene Catabolism Operon in the Psychrotrophic Strain Pseudomonas putida 01G3-Reference-Cited by-同舟云学术

Genetic and Molecular Organization of the Alkylbenzene Catabolism Operon in the Psychrotrophic Strain Pseudomonas putida 01G3

Published:2001-01 Issue:1 Volume:67 Page:453-458
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Chablain P. A.¹,Zgoda A. L.²,Sarde C.-O.²,Truffaut N.¹

Affiliation:

1. Laboratoire de Génétique Microbienne1 and

2. Laboratoire de Technologie Enzymatique,2 UMR 6022 CNRS, Université de Technologie de Compiègne, 60205 Compiègne cedex, France

Abstract

ABSTRACT The 11-kb sequence encompassing the alkylbenzene upper pathway in Pseudomonas putida 01G3, a psychrotrophic strain able to degrade alkylbenzenes at low temperatures, was characterized. Together with a potential regulator (EbdR), six putative enzymes (EbdAaAbAcAdBC) were identified, and they exhibited highly significant similarities with enzymes implicated in the equivalent pathway in P. putida RE204. ebd genes appeared to be preferentially induced by ethylbenzene. Multiple-alignment data and growth rate measurements led us to classify 01G3 and closely related strains in two groups with distinct substrate specificities. Close to identified genes, remnants of IS 5 -like elements provided insight into the evolution of catabolic sequences through rearrangements from a less complex ancestral cluster.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.1.453-458.2001

Reference28 articles.

1. Cloning, nucleotide sequence and characterization of the genes encoding the enzymes involved in the degradation of cumene to 2-hydroxy-6-oxo-7-methylocta-2,4-dienoic acid in Pseudomonas fluorescens IP01.;Aoki H.;J. Ferment. Bioeng.,1996

2. Complete nucleotide sequence and evolutionary significance of a chromosomally encoded naphthalene-degradation lower pathway from Pseudomonas stutzeri AN10.;Bosch R.;Gene,2000

3. Genetic characterization and evolutionary implications of a chromosomally encoded naphthalene-degradation upper pathway from Pseudomonas stutzeri AN10.;Bosch R.;Gene,1999

4. The helix-turn-helix DNA binding motif.;Brennan R. G.;J. Biol. Chem.,1989

5. Isolation of a soil psychrotrophic toluene-degrading Pseudomonas strain: influence of temperature on the growth characteristics on different substrates.;Chablain P. A.;Res. Microbiol.,1997

Cited by 17 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Isolation and Characterization a Novel Catabolic Gene Cluster Involved in Chlorobenzene Degradation in Haloalkaliphilic Alcanivorax sp. HA03;Biology;2022-05-09

2. Functional characterization of diverse ring-hydroxylating oxygenases and induction of complex aromatic catabolic gene clusters inSphingobiumsp. PNB;FEBS Open Bio;2014-01-01

3. Cloning of dfdA genes from Terrabacter sp. strain DBF63 encoding dibenzofuran 4,4a-dioxygenase and heterologous expression in Streptomyces lividans;Applied Microbiology and Biotechnology;2012-11-23

4. An insight into the origin and functional evolution of bacterial aromatic ring-hydroxylating oxygenases;Journal of Biomolecular Structure and Dynamics;2012-06-12

5. Functional adaptation of microbial communities from jet fuel-contaminated soil under bioremediation treatment: simulation of pollutant rebound;FEMS Microbiology Ecology;2011-08-01