Lyme Disease Borrelia spp. in Ticks and Rodents from Northwestern China

Author:

Takada Nobuhiro1,Masuzawa Toshiyuki2,Ishiguro Fubito3,Fujita Hiromi4,Kudeken Midori2,Mitani Harumi5,Fukunaga Masahito5,Tsuchiya Kimiyuki6,Yano Yasuhiro1,Ma Xiao-Hang7

Affiliation:

1. Department of Immunology and Medical Zoology, Fukui Medical University, Matsuoka, Fukui 910-1193,1

2. Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526,2

3. Fukui Prefectural Institute of Public Health, Fukui 910-8551,3

4. Research Laboratory of Ohara General Hospital, Fukushima 960-0195,4

5. Laboratory of Molecular Microbiology, Faculty of Pharmacy and Pharmaceutical Science, Fukuyama University, Fukuyama 729-0292,5 and

6. Experimental Animal Center, Miyazaki Medical College, Kiyotake 889-1692,6 Japan; and

7. Department of Microbiology, Faculty of Life Science, Zhejiang University, Hangzhou, People's Republic of China7

Abstract

ABSTRACT In May 1999, field surveys of Lyme disease spirochetes were conducted around the Tianshan Mountains in Xinjiang Uygur Autonomous Region in northwestern People's Republic of China. Ixodes persulcatus ticks were obtained in a Tianchi Lake valley with primary forest, while the tick fauna was poor in the semidesert or at higher altitudes in this region. Species identities were confirmed by molecular analysis in which an internal transcribed spacer sequence was used. Of 55 adult ticks, 22 (40%) were positive for spirochetes as determined by Barbour-Stoenner-Kelly culture passages. In addition, some rodents, including Apodemus uralensis (5 of 14 animals) and Cricetulus longicaudatus (the only animal examined), and some immature stages of I. persulcatus (4 of 11 ticks) that had fed on A. uralensis were positive for spirochetes. Based on 5S-23S rRNA intergenic spacer restriction fragment length polymorphism analysis and reactivity with monoclonal antibodies, 35 cultures (including double isolation cultures) were identified as Borrelia garinii (20 isolates, including 9 Eurasian pattern B isolates and 11 Asian pattern C isolates), Borrelia afzelii (10 pattern D isolates), and mixed cultures (5 cultures, including isolates that produced B. garinii patterns B and C plus B. afzelii pattern D). These findings revealed that Lyme disease pathogens are distributed in the mountainous areas in northwestern China even though it is an arid region, and they also confirmed the specific relationship between I. persulcatus and genetic patterns of Borrelia spp. on the Asian continent.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference27 articles.

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2. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis.;Baranton G.;Int. J. Syst. Bacteriol.,1992

3. Isolation of a cultivable spirochete from I. ricinus of Switzerland.;Barbour A. G.;Curr. Microbiol.,1983

4. Editorial Committee of the Distribution Mammalian Species in China Distribution of mammalian species in China 1997 251 China Forestry Publishing House Beijing People's Republic of China(In Chinese.)

5. Filippova N. A. Taiga tick Ixodes persulcatus (Acarina: Ixodidae). Morphplogy systematics ecology medical importance 1985 416 Soviet Committee for the UNESCO Programme Man and Biosphere Leningrad Russia (In Russian.)

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