Poly(3-Hydroxybutyrate) Synthesis Genes in Azotobacter sp. Strain FA8

Author:

Pettinari M. Julia1,Vázquez Gustavo J.1,Silberschmidt Daniel1,Rehm Bernd2,Steinbüchel Alexander2,Méndez Beatriz S.1

Affiliation:

1. Departamento de Quı́mica Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina,1and

2. Institut für Mikrobiologie, Westfälische Wilhems-Universität Münster, D-48149 Münster, Germany2

Abstract

ABSTRACT Genes responsible for the synthesis of poly(3-hydroxybutyrate) (PHB) in Azotobacter sp. FA8 were cloned and analyzed. A PHB polymerase gene ( phbC ) was found downstream from genes coding for β-ketothiolase ( phbA ) and acetoacetyl-coenzyme A reductase ( phbB ). A PHB synthase mutant was obtained by gene inactivation and used for genetic studies. The phbC gene from this strain was introduced into Ralstonia eutropha PHB-4 ( phbC -negative mutant), and the recombinant accumulated PHB when either glucose or octanoate was used as a source of carbon, indicating that this PHB synthase cannot incorporate medium-chain-length hydroxyalkanoates into PHB.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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