Assessing the Diversity of Marine Bacterial β-Glucosidases by Capillary Electrophoresis Zymography-Reference-Cited by-同舟云学术

Assessing the Diversity of Marine Bacterial β-Glucosidases by Capillary Electrophoresis Zymography

Published:2001-10 Issue:10 Volume:67 Page:4896-4900
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Arrieta Jesús M.¹,Herndl Gerhard J.¹

Affiliation:

1. Department of Biological Oceanography, Netherlands Institute for Sea Research, 1790 AB Den Burg, Texel, The Netherlands

Abstract

ABSTRACT We propose a new method for the fast separation and detection of β-glucosidases in environmental samples. With this approach, β-glucosidases extracted from bacteria are evidenced by substrate-incorporated capillary electrophoresis (CE zymography) and their kinetic parameters can be determined by repeated injections using different substrate concentrations. Preliminary results obtained with natural bacterial communities from the coastal North Sea suggest that the diversity of β-glucosidases in the marine environment might be much higher than previously observed.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.67.10.4896-4900.2001

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