Prevalence and Characteristics of Shiga Toxin-Producing Escherichia coli from Healthy Cattle in Japan

Author:

Kobayashi Hideki12,Shimada Jun3,Nakazawa Muneo1,Morozumi Tetsuo1,Pohjanvirta Tarja2,Pelkonen Sinikka2,Yamamoto Koshi1

Affiliation:

1. National Institute of Animal Health, 3-1-1, Kannondai, Tsukuba 305-0856,1 and

2. National Veterinary and Food Research Institute, Kuopio Regional Laboratory, FIN-70701, Kuopio, Finland2

3. Chiba Prefectural Institute of Animal Health, 497, Iwatomi, Sakura 285-0072,3 Japan, and

Abstract

ABSTRACT The prevalence of Shiga toxin-producing Escherichia coli (STEC) in Japan was examined by using stool samples from 87 calves, 88 heifers, and 183 cows on 78 farms. As determined by screening with stx -PCR, the prevalence was 46% in calves, 66% in heifers, and 69% in cows; as determined by nested stx -PCR, the prevalence was 100% in all animal groups. Of the 962 isolates picked by colony stx hybridization, 92 isolates from 54 farms were characterized to determine their O serogroups, virulence factor genes, and antimicrobial resistance. Of these 92 isolates, 74 (80%) could be classified into O serogroups; 50% of these 74 isolates belonged to O serogroups O8, O26, O84, O113, and O116 and 1 isolate belonged to O serogroup O157. Locus of enterocyte effacement genes were detected in 24% of the isolates, and enterohemorrhagic E. coli (EHEC) hlyA genes were detected in 72% of the isolates. Neither the bundle-forming pilus gene nor the enteropathogenic E. coli adherence factor plasmid was found. STEC strains with characteristics typical of isolates from human EHEC infections, which were regarded as potential EHEC strains, were present on 11.5% of the farms.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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