Affiliation:
1. National Institute of Animal Health, 3-1-1, Kannondai, Tsukuba 305-0856,1 and
2. National Veterinary and Food Research Institute, Kuopio Regional Laboratory, FIN-70701, Kuopio, Finland2
3. Chiba Prefectural Institute of Animal Health, 497, Iwatomi, Sakura 285-0072,3 Japan, and
Abstract
ABSTRACT
The prevalence of Shiga toxin-producing
Escherichia coli
(STEC) in Japan was examined by using stool samples from 87 calves, 88 heifers, and 183 cows on 78 farms. As determined by screening with
stx
-PCR, the prevalence was 46% in calves, 66% in heifers, and 69% in cows; as determined by nested
stx
-PCR, the prevalence was 100% in all animal groups. Of the 962 isolates picked by colony
stx
hybridization, 92 isolates from 54 farms were characterized to determine their O serogroups, virulence factor genes, and antimicrobial resistance. Of these 92 isolates, 74 (80%) could be classified into O serogroups; 50% of these 74 isolates belonged to O serogroups O8, O26, O84, O113, and O116 and 1 isolate belonged to O serogroup O157. Locus of enterocyte effacement genes were detected in 24% of the isolates, and enterohemorrhagic
E. coli
(EHEC)
hlyA
genes were detected in 72% of the isolates. Neither the bundle-forming pilus gene nor the enteropathogenic
E. coli
adherence factor plasmid was found. STEC strains with characteristics typical of isolates from human EHEC infections, which were regarded as potential EHEC strains, were present on 11.5% of the farms.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
139 articles.
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