Affiliation:
1. Institute of Microbiology, Russian Academy of Sciences, Moscow 117811, Russia,1 and
2. Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany2
Abstract
ABSTRACT
Two 16S rRNA-targeted oligonucleotide probes, Mcell-1026 and Mcell-181, were developed for specific detection of the acidophilic methanotroph
Methylocella palustris
using fluorescence in situ hybridization (FISH). The fluorescence signal of probe Mcell-181 was enhanced by its combined application with the oligonucleotide helper probe H158. Mcell-1026 and Mcell-181, as well as 16S rRNA oligonucleotide probes with reported group specificity for either type I methanotrophs (probes M-84 and M-705) or the
Methylosinus
/
Methylocystis
group of type II methanotrophs (probes MA-221 and M-450), were used in FISH to determine the abundance of distinct methanotroph groups in a
Sphagnum
peat sample of pH 4.2.
M. palustris
was enumerated at greater than 10
6
cells per g of peat (wet weight), while the detectable population size of type I methanotrophs was three orders of magnitude below the population level of
M. palustris
. The cell counts with probe MA-221 suggested that only 10
4
type II methanotrophs per g of peat (wet weight) were present, while the use of probe M-450 revealed more than 10
6
type II methanotroph cells per g of the same samples. This discrepancy was due to the fact that probe M-450 targets almost all currently known strains of
Methylosinus
and
Methylocystis
, whereas probe MA-221, originally described as group specific, does not detect a large proportion of
Methylocystis
strains. The total number of methanotrophic bacteria detected by FISH was 3.0 (±0.2) × 10
6
cells per g (wet weight) of peat. This was about 0.8% of the total bacterial cell number. Thus, our study clearly suggests that
M. palustris
and a defined population of
Methylocystis
spp. were the predominant methanotrophs detectable by FISH in an acidic
Sphagnum
peat bog.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
128 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献