Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens-Reference-Cited by-同舟云学术

Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens

Published:2015-11 Issue:11 Volume:53 Page:3606-3608
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Peterson S. W.¹,Martin I.¹,Demczuk W.¹,Bharat A.¹,Hoang L.²,Wylie J.³,Allen V.⁴,Lefebvre B.⁵,Tyrrell G.⁶,Horsman G.⁷,Haldane D.⁸,Garceau R.⁹,Wong T.¹⁰,Mulvey M. R.¹¹¹

Affiliation:

1. Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

2. British Columbia Centres for Disease Control Public Health Microbiology & Reference Laboratory, Vancouver, British Columbia, Canada

3. Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada

4. Public Health Ontario Laboratories, Toronto, Ontario, Canada

5. Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada

6. Provincial Laboratory for Public Health, Edmonton, Alberta, Canada

7. Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada

8. Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada

9. Dr. G. L. Dumont Hospital, Moncton, New Brunswick, Canada

10. Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada, Ottawa, Ontario, Canada

11. Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada

Abstract

ABSTRACT We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs was sufficient to predict ciprofloxacin resistance in an organism.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01632-15

Reference12 articles.

1. Public Health Agency of Canada. 2014. Notifiable diseases on-line. Public Health Agency of Canada, Ottawa, Ontario, Canada. http://dsol-smed.phac-aspc.gc.ca/dsol-smed/ndis/charts.php?c=pl.

2. The use of cephalosporins for gonorrhea: the impending problem of resistance

3. Public Health Agency of Canada, National Microbiology Laboratory. 2014. National surveillance of antimicrobial susceptibilities of Neisseria gonorrhoeae annual summary 2012. Public Health Agency of Canada, Ottawa, Ontario, Canada. http://publications.gc.ca/collections/collection_2014/aspc-phac/HP57-3-2012-eng.pdf.

4. Neisseria gonorrhoeaeacquires mutations in analogous regions of gyrA and parC in fluoroquinolone‐resistant isolates

5. Molecular Assay for Detection of Genetic Markers Associated with Decreased Susceptibility to Cephalosporins in Neisseria gonorrhoeae

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