Affiliation:
1. U.S. Army Chemical Corps Biological Laboratories, Fort Detrick, Frederick, Maryland
Abstract
McDonald, William
C. (U.S. Army Chemical Corps, Fort Detrick, Frederick, Md.)
and Thomas S. Matney
. Genetic transfer of the ability to grow at 55 C in
Bacillus subtilis
. J. Bacteriol.
85:
218–220. 1962.—Two strains of
Bacillus subtilis
, 168 and P1, were found to grow at 55 C (55
+
) on complete media; strain 168S
r
failed to grow at temperatures above 50 C (55
−
). When 168S
r
bacteria were grown in the presence of deoxyribonucleic acid extracted from 168, the ability to grow at 55 C was transformed at a frequency of 10
−4
. An incubation period of 3 to 4 hr at 37 C was necessary for phenotypic expression of the 55
+
character. Only 10 to 20% of the 55
+
transformants retained the high-level streptomycin resistance (S
r
) of the recipient, indicating close linkage between the S
s
and 55
+
loci.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference6 articles.
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5. Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate;SPIZIZEN J.;Proc. Natl. Acad. Sci. U.,1958
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