Author:
Benedik M,Fennewald M,Shapiro J
Abstract
The beta-lactamase gene from the RP1 plasmid transposes into at least two Pseudomonas putida degradative plasmids. Donor strains that carry RP1 (bla+ tet+ aphA+) and a degradative plasmid yield transconjugants that have only the bla+ marker of RP1. This occurs in up to 80% of all bla+ transconjugants. Segregation of the bla+ marker requires the presence of a degradative plasmid in the donor and is only observed in transconjugants that have received degradative markers. The bla+ tet aphA transconjugants show 100% linkage of bla+ to degradative markers in conjugation,transduction, and transformation crosses. A transduction cross of an (RP1), (SAL) donor shows that 8% of all SAL plasmids also carry the transposed bla+ marker. Tn401 is the name we assign to the bla+ transposon from RP1 observed in Pseudomonas. Its identity with the RP1 bla+ transposon observed in Escherichia coli is not known. In four cases, Tn401 has inserted into the camphor genes of the CAM-OCT plasmid.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference23 articles.
1. Translocation of a discrete piece of deoxyribonucleic acid carrying an amp gene between replicons in Escherichia coli;Bennett P. M.;J. Bacteriol.,1976
2. Plasmid-determined alcohol dehydrogenase activity in alkane-utilizing strains ofPseudomonas putida;Benson S.;J. Bacteriol.,1976
3. Genetic analysis of amidase mutants of Pseudomonas aeruginosa;Betz J. L.;Genet. Res.,1974
4. Bryan L. E. and M. S. Shahrabadi. 1975. Properties of P-2 R factors of Pseudomonas aeruginosa some of which mediate gentamycin resistance p. 53-55. In D. Schlessinger (ed.) Microbiology- 1974. American Society for Microbiology Washington D.C.
5. Genetic basis ofthe biodegradation of salicylate in Pseudomonas;Chakrabarty A. M.;J. Bacteriol.,1972
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