Multicenter Performance Evaluation of the Simplexa Bordetella Direct Kit in Nasopharyngeal Swab Specimens-Reference-Cited by-同舟云学术

Multicenter Performance Evaluation of the Simplexa Bordetella Direct Kit in Nasopharyngeal Swab Specimens

Published:2020-12-17 Issue:1 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Chow Siu-Kei¹^ORCID,Arbefeville Sophie²,Boyanton Bobby L.³,Dault Emily M.³,Dunn James⁴,Ferrieri Patricia²,Greene Wallace⁵,Pence Morgan A.⁶^ORCID,Otiso Joshua⁷,Richter Sandra⁷,Schutzbank Ted E.⁸

Affiliation:

1. Department of Microbiology, Laboratories Northwest, MultiCare Health System, Tacoma, Washington, USA

2. Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota, USA

3. Department of Pathology and Laboratory Medicine, Beaumont Health, Royal Oak, Michigan, USA

4. Department of Pathology, Texas Children’s Hospital, Houston, Texas, USA

5. Department of Pathology and Laboratory Medicine, Penn State Health Milton S. Hershey Medical Center, Hershey, Pennsylvania, USA

6. Cook Children’s Medical Center, Fort Worth, Texas, USA

7. Department of Laboratory Medicine, Cleveland Clinic, Cleveland, Ohio, USA

8. Ascension St. John Hospital, Detroit, Michigan, USA

Abstract

Detection of Bordetella pertussis and Bordetella parapertussis using molecular methods is sensitive and specific with a short turnaround time compared to other diagnostic methods. In this multicenter study, we compared the performance of the Simplexa Bordetella Direct kit to those of other molecular assays in detecting and differentiating B. pertussis and B. parapertussis in nasopharyngeal swab specimens. The limits of detection (LODs) were 150 CFU/ml or 3 fg/μl of DNA for B. pertussis and 1,500 CFU/ml or 10 fg/μl of DNA for B. parapertussis .

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01041-20

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