Capturing the Resistome: a Targeted Capture Method To Reveal Antibiotic Resistance Determinants in Metagenomes-Reference-Cited by-同舟云学术

Capturing the Resistome: a Targeted Capture Method To Reveal Antibiotic Resistance Determinants in Metagenomes

Published:2019-12-20 Issue:1 Volume:64 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Guitor Allison K.¹²³,Raphenya Amogelang R.¹²³,Klunk Jennifer³⁴,Kuch Melanie³⁴,Alcock Brian¹²³,Surette Michael G.¹²³,McArthur Andrew G.¹²³^ORCID,Poinar Hendrik N.¹²³⁴,Wright Gerard D.¹²³

Affiliation:

1. David Braley Centre for Antibiotic Discovery, McMaster University, Hamilton, Ontario, Canada

2. Michael G. DeGroote Institute for Infectious Disease Research, McMaster University, Hamilton, Ontario, Canada

3. Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, Ontario, Canada

4. McMaster Ancient DNA Centre, Department of Anthropology and Biochemistry, McMaster University, Hamilton, Ontario, Canada

Abstract

Identification of the nucleotide sequences encoding antibiotic resistance elements and determination of their association with antibiotic resistance are critical to improve surveillance and monitor trends in antibiotic resistance. Current methods to study antibiotic resistance in various environments rely on extensive deep sequencing or laborious culturing of fastidious organisms, both of which are heavily time-consuming operations.

Funder

Canadian Foundation for Innovation

Boris Family Foundation

Ontario Graduate Scholarship

Canada Research Chairs

Gouvernement du Canada | Canadian Institutes of Health Research

Genome Canada

Cisco Systems

Ontario Research Foundation

Publisher

American Society for Microbiology

Subject