Effects of Spatial Variability and Relic DNA Removal on the Detection of Temporal Dynamics in Soil Microbial Communities

Author:

Carini Paul1ORCID,Delgado-Baquerizo Manuel12,Hinckley Eve-Lyn S.34,Holland‐Moritz Hannah15,Brewer Tess E.1,Rue Garrett3,Vanderburgh Caihong1,McKnight Diane3,Fierer Noah15ORCID

Affiliation:

1. Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, Colorado, USA

2. Departamento de Biología y Geología, Física y Química Inorgánica, Escuela Superior de Ciencias Experimentales, Universidad Rey Juan Carlos, Móstoles, Spain

3. Institute of Arctic and Alpine Research, University of Colorado, Boulder, Colorado, USA

4. Environmental Studies Program, University of Colorado, Boulder, Colorado, USA

5. Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, Colorado, USA

Abstract

Nearly all microbial communities are dynamic in time. Understanding how temporal dynamics in microbial community structure affect soil biogeochemistry and fertility are key to being able to predict the responses of the soil microbiome to environmental perturbations. Here, we explain the effects of soil spatial structure and relic DNA on the determination of microbial community fluctuations over time. We found that intensive spatial sampling was required to identify temporal effects in microbial communities because of the high degree of spatial heterogeneity in soil and that DNA from nonliving sources masks important temporal patterns. We identified groups of microbes with shared temporal responses and show that these patterns were predictable from changes in soil characteristics. These results provide insight into the environmental preferences and temporal relationships between individual microbial taxa and highlight the importance of considering relic DNA when trying to detect temporal dynamics in belowground communities.

Funder

University of Colorado Cooperative Institute for Environmental Sciences

National Science Foundation

Publisher

American Society for Microbiology

Subject

Virology,Microbiology

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3