Localization of the Escherichia coli RNA Polymerase β′ Subunit Residue Phosphorylated by Bacteriophage T7 Kinase Gp0.7-Reference-Cited by-同舟云学术

Localization of the Escherichia coli RNA Polymerase β′ Subunit Residue Phosphorylated by Bacteriophage T7 Kinase Gp0.7

Published:2006-05-15 Issue:10 Volume:188 Page:3470-3476
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Severinova Elena¹,Severinov Konstantin¹²³

Affiliation:

1. Waksman Institute, Piscataway, New Jersey 08854

2. Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey 08854

3. Institute of Molecular Genetics, Russian Academy of Sciences, Moscow 142292, Russia

Abstract

ABSTRACT During bacteriophage T7 infection, the Escherichia coli RNA polymerase β′ subunit is phosphorylated by the phage-encoded kinase Gp0.7. Here, we used proteolytic degradation and mutational analysis to localize the phosphorylation site to a single amino acid, Thr 1068 , in the evolutionarily hypervariable segment of β′. Using a phosphomimetic substitution of Thr 1068 , we show that phosphorylation of β′ leads to increased ρ-dependent transcription termination, which may help to switch from host to viral RNA polymerase transcription during phage development.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.188.10.3470-3476.2006

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