Single-Point Mutations Causing More than 100-Fold Underestimation of Human Immunodeficiency Virus Type 1 (HIV-1) Load with the Cobas TaqMan HIV-1 Real-Time PCR Assay-Reference-Cited by-同舟云学术

Single-Point Mutations Causing More than 100-Fold Underestimation of Human Immunodeficiency Virus Type 1 (HIV-1) Load with the Cobas TaqMan HIV-1 Real-Time PCR Assay

Published:2009-04 Issue:4 Volume:47 Page:1238-1240
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Korn Klaus¹,Weissbrich Benedikt²,Henke-Gendo Cornelia³,Heim Albert³,Jauer Christin Mariel⁴,Taylor Ninon⁵,Eberle Josef⁴

Affiliation:

1. Institute of Clinical and Molecular Virology, National Reference Center for Retroviruses, University of Erlangen-Nuremberg, Erlangen, Germany

2. Institute of Virology and Immunobiology, University of Würzburg, Würzburg, Germany

3. Institute of Virology, Medical School Hannover, Hannover, Germany

4. Max von Pettenkofer-Institute, Ludwig-Maximilians-University, Munich, Germany

5. Third Medical Department, Paracelsus Medical University of Salzburg, Salzburg, Austria

Abstract

ABSTRACT Systematic sequence analysis of human immunodeficiency virus type 1 (HIV-1) variants with RNA levels underestimated by the Cobas TaqMan HIV-1 assay demonstrated that mutations at a single position of the downstream primer can lead to the underestimation of HIV-1 RNA levels by more than 2 log and to false-negative results in minipool screening of blood donors. Mutations at this position are found in about 2% of all HIV-1 M gag sequences.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02204-08

Reference3 articles.

1. Colson, P., C. Solas, J. Moreau, A. Motte, M. Henry, and C. Tamalet. 2007. Impaired quantification of plasma HIV-1 RNA with a commercialized real-time PCR assay in a couple of HIV-1-infected individuals. J. Clin. Virol.39:226-229.

2. Human Immunodeficiency Virus Type 1 (HIV-1) Plasma Load Discrepancies between the Roche COBAS AMPLICOR HIV-1 MONITOR Version 1.5 and the Roche COBAS AmpliPrep/COBAS TaqMan HIV-1 Assays

3. Rates of and Reasons for Failure of Commercial Human Immunodeficiency Virus Type 1 Viral Load Assays in Brazil

Cited by 49 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Analysis of the Frequency of Mutations at Diagnostic Oligonucleotide Sites and Their Impact on the Efficiency of PCR for HIV-1;Microorganisms;2023-11-22

2. Performance assessment of the new Xpert® HIV-1 viral load XC assay for quantification of HIV-1 viral loads;Journal of Clinical Virology;2022-04

3. SARS-CoV-2 N gene mutations impact detection by clinical molecular diagnostics: reports in two cities in the United States;Diagnostic Microbiology and Infectious Disease;2021-11

4. A low-cost in-house HIV integrase strand transfer inhibitor drug resistance test for resource-limited settings;F1000Research;2021-03-31

5. Severe underquantification of HIV-1 group O isolates by major commercial PCR-based assays;Clinical Microbiology and Infection;2020-12