Disruption of Spermatogenic Cell Adhesion and Male Infertility in Mice Lacking TSLC1/IGSF4, an Immunoglobulin Superfamily Cell Adhesion Molecule
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Published:2006-05
Issue:9
Volume:26
Page:3610-3624
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ISSN:0270-7306
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Container-title:Molecular and Cellular Biology
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language:en
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Short-container-title:Mol Cell Biol
Author:
Yamada Daisuke1, Yoshida Midori2, Williams Yuko N.1, Fukami Takeshi1, Kikuchi Shinji1, Masuda Mari1, Maruyama Tomoko1, Ohta Tsutomu3, Nakae Dai2, Maekawa Akihiko2, Kitamura Tadaichi4, Murakami Yoshinori1
Affiliation:
1. Tumor Suppression and Functional Genomics Project 2. Department of Pathology, Sasaki Institute, Sasaki Foundation, Tokyo, Japan 3. Genetics Division, National Cancer Center Research Institute, Tokyo, Japan 4. Department of Urology, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
Abstract
ABSTRACT
TSLC1/IGSF4, an immunoglobulin superfamily molecule, is predominantly expressed in the brain, lungs, and testes and plays important roles in epithelial cell adhesion, cancer invasion, and synapse formation. We generated
Tslc1/Igsf4
-deficient mice by disrupting exon 1 of the gene and found that
Tslc1
−/−
mice were born with the expected Mendelian ratio but that
Tslc1
−/−
male mice were infertile. In 11-week-old adult
Tslc1
−/−
mice, the weight of a testis was 88% that in
Tslc1
+/+
mice, and the number of sperm in the semen was approximately 0.01% that in
Tslc1
+/+
mice. Histological analysis revealed that the round spermatids and the pachytene spermatocytes failed to attach to the Sertoli cells in the seminiferous tubules and sloughed off into the lumen with apoptosis in the
Tslc1
−/−
mice. On the other hand, the spermatogonia and the interstitial cells, including Leydig cells, were essentially unaffected. In the
Tslc1
+/+
mice, TSLC1/IGSF4 expression was observed in the spermatogenic cells from the intermediate spermatogonia to the early pachytene spermatocytes and from spermatids at step 7 or later. These findings suggest that TSLC1/IGSF4 expression is indispensable for the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Reference20 articles.
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