Use of the Genomic Subtractive Hybridization Technique To Develop a Real-Time PCR Assay for Quantitative Detection of Prevotella spp. in Oral Biofilm Samples-Reference-Cited by-同舟云学术

Use of the Genomic Subtractive Hybridization Technique To Develop a Real-Time PCR Assay for Quantitative Detection of Prevotella spp. in Oral Biofilm Samples

Published:2005-06 Issue:6 Volume:43 Page:2948-2951
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Nagashima Shiori¹,Yoshida Akihiro¹,Suzuki Nao²,Ansai Toshihiro¹,Takehara Tadamichi¹

Affiliation:

1. Division of Community Oral Health Science, Kyushu Dental College, Kitakyushu 803-8580

2. Department of Oral Bacteriology, Ohu University School of Dentistry, Koriyama 963-8611, Japan

Abstract

ABSTRACT Genomic subtractive hybridization was used to design Prevotella nigrescens -specific primers and TaqMan probes. Based on this technique, a TaqMan real-time PCR assay was developed for quantifying four oral black-pigmented Prevotella species. The combination of real-time PCR and genomic subtractive hybridization is useful for preparing species-specific primer-probe sets for closely related species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.43.6.2948-2951.2005

Reference22 articles.

1. Akopyants, N. S., A. Fradkov, L. Diatchenko, J. E. Hill, P. D. Siebert, S. A. Lukyanov, E. D. Sverdlov, and D. E. Berg. 1998. PCR-based subtractive hybridization and differences in gene content among strains of Helicobacter pylori. Proc. Natl. Acad. Sci. USA95:13108-13113.

2. Cloning and characterization of a Prevotella melaninogenica hemolysin

3. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs

4. Ansai, T., S. Awano, X. Chen, T. Fuchi, T. Arimoto, S. Akifusa, and T. Takehara. 1998. Purification and characterization of alkaline phosphatase containing phosphotyrosyl phosphatase activity from the bacterium Prevotella intermedia. FEBS Lett.428:157-160.

5. Isolation, Cloning, and Expression of an Acid Phosphatase Containing Phosphotyrosyl Phosphatase Activity from Prevotella intermedia

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