Xer Site-Specific Recombination, an Efficient Tool To Introduce Unmarked Deletions into Mycobacteria-Reference-Cited by-同舟云学术

Xer Site-Specific Recombination, an Efficient Tool To Introduce Unmarked Deletions into Mycobacteria

Published:2010-08 Issue:15 Volume:76 Page:5312-5316
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Cascioferro Alessandro¹,Boldrin Francesca¹,Serafini Agnese¹,Provvedi Roberta²,Palù Giorgio¹,Manganelli Riccardo¹

Affiliation:

1. Department of Histology, Microbiology, and Medical Biotechnologies

2. Department of Biology, University of Padua, Padua, Italy

Abstract

ABSTRACT Genetic manipulation of mycobacteria still represents a serious challenge due to the lack of tools and selection markers. In this report, we describe the development of an intrinsically unstable excisable cassette for introduction of unmarked mutations in both Mycobacterium smegmatis and Mycobacterium tuberculosis .

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00382-10

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