Kinetics of the Vaccinia Virus Plaque Neutralization Test

Author:

O'Brien Thomas C.1,Myers Martin G.1,Tauraso Nicola M.1

Affiliation:

1. Laboratory of Virology and Rickettsiology, Division of Biologics Standards, National Institutes of Health, Bethesda, Maryland 20014

Abstract

Neutralization of vaccinia virus with immune rabbit serum occurred optimally when incubated at 37 C for 16 to 24 hr in the plaque neutralization test employing the MA-104 embryonic rhesus monkey kidney cell line.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference26 articles.

1. A simplified plaque technique for titration of pox virus and its antibodies in chick embryo cells;Anderson E. K.;Acta Pathol. Microbiol. Scand.,1966

2. Anderson S. G. and J. Skegg. 1970. The international standard anti-smallpox serum. Bull. W. H. 0. 42:515-522.

3. Antivaccinial serum. 3. Evidence for slow union with virus in vitro;Andrewes C. H.;J. Pathol. Bacteriol.,1930

4. The action of immune serum on vaccinia and virus III in vitro;Andrewes C. H.;J. Pathol. Bacteriol.,1928

5. The titration of vaccinial neutralizing antibody on chorio-allantoic membranes;Boulter E. A.;J. Hyg.,1957

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