Abstract
This study evaluated the ability of the RapID-ANA system (Innovative Diagnostic Systems, Inc., Atlanta, Ga.) to accurately identify a spectrum of freshly isolated veterinary anaerobes. A total of 183 isolates were tested and included 7 Actinomyces spp., 53 Bacteroides spp., 32 Clostridium spp., 2 Eubacterium spp., 65 Fusobacterium spp., 1 Peptococcus spp., 22 Peptostreptococcus spp., and 1 Propionibacterium spp. All isolates were initially identified by conventional biochemical testing and gas-liquid chromatography of short-chain fatty acid metabolites. Additional tests were performed as required by the RapID-ANA system. Of these isolates, 81.4% were correctly identified to the genus level, including 59.6% to the species level, 14.2% were incorrectly identified at the genus level, and 4.4% were not identified. Initially, 20.2% of the strains were not identified because the microcodes were not in the code book. The majority of the incorrect identifications were caused by the misidentification of Fusobacterium spp. as Bacteroides spp. Errors also occurred when veterinary anaerobes not included in the data base were assigned an identification from the existing data base. The RapID-ANA system appears to be a promising new method for rapid identification of veterinary anaerobes; however, further evaluation with an extended data base is needed before the system can accurately identify all clinically significant anaerobes.
Publisher
American Society for Microbiology
Reference14 articles.
1. Alen S. D. J. Â. Siders and L. M. Marler. 1985. Isolation and identification of anaerobic bacteria p. 413-433. In E. H. Lennette A. Balows W. J. Hausler Jr. and H. J. Shadomy (ed.) Manual of clinical microbiology 4th ed. American Society for Microbiology Washington D.C.
2. Accuracy and reproducibility of a four-hour method for anaerobe identification;Appelbaum P. C.;J. Clin. Microbiol.,1985
3. Occurrence of anaerobic bacteria in diseases of the dog and cat;Berg J. N.;Am. J. Vet. Res.,1979
4. Isolation and identification of anaerobes in the veterinary diagnostic laboratory;Berkhoff G. A.;Am. J. Vet. Res.,1977
5. Actinomyces hordeovulneris sp. nov., an agent of canine actinomycosis;Buchanan A. M.;Int. J. Syst. Bacteriol.,1984
Cited by
18 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献