New Efficient Recombinant Expression System To Engineer Candida antarctica Lipase B-Reference-Cited by-同舟云学术

New Efficient Recombinant Expression System To Engineer Candida antarctica Lipase B

Published:2010-04-15 Issue:8 Volume:76 Page:2684-2687
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Emond Stéphane¹,Montanier Cédric¹,Nicaud Jean-Marc²,Marty Alain¹,Monsan Pierre¹³,André Isabelle¹,Remaud-Siméon Magali¹

Affiliation:

1. Ingénierie des Systèmes Biologiques et des Procédés, Université de Toulouse, INSA, UPS, INP, 135 Avenue de Rangueil, F-31077 Toulouse, France, and INRA, UMR792, F-31400 Toulouse, France

2. Laboratoire de Microbiologie et Génétique Moléculaire, INRA, UMR1238, CNRS, UMR2585, AgroParisTech, F-78850 Thieverval-Grignon, France

3. Institut Universitaire de France, 103 Boulevard Saint-Michel, F-75005 Paris, France

Abstract

ABSTRACT Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.03057-09

Reference29 articles.

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3. Bordes, F., F. Fudalej, V. Dossat, J. M. Nicaud, and A. Marty. 2007. A new recombinant protein expression system for high-throughput screening in the yeast Yarrowia lipolytica. J. Microbiol. Methods70:493-502.

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