Abstract
Antibodies in both mouse and human Rickettsia mooseri (Rickettsia typhi) convalescent serum that were cytophilic for mouse macrophages were demonstrated by the rosette technique. Mouse peritoneal macrophages, passively sensitized with early and late serum from mice with a sublethal infection of R. mooseri, were washed and exposed to rickettsiae. Rosettes of rickettsiae were found around macrophages, maintained at 4 degrees C, which had been sensitized with immune serum (direct sensitization of macrophages), but no rosettes were found around macrophages sensitized with serum from normal mice. When the macrophages were maintained at 34 degrees C after addition of the rickettsiae, phagocytosis of rickettsiae occurred, indicating one probable role for cytophilic antibodies in typhus infections. If the rickettsiae were mixed with serum from infected mice, washed, and then added to macrophages (indirect sensitization of macrophages), more rosettes were found around the macrophages than around directly sensitized macrophages. The presence of mouse immunoglobulin G on the macrophage surface was also shown by staining living sensitized macrophages with rabbit fluroescein-conjugated anti-mouse immunoglobulin G.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献