Isolation of a factor causing morphological changes of chinese hamster ovary cells from the culture filtrate of Vibrio parahaemolyticus

Author:

Honda T,Shimizu M,Takeda Y,Miwatani T

Abstract

A factor changing Chinese hamster ovary cells from an oval to a spindle shape was isolated from the culture filtrate of Vibrio parahaemolyticus. It was partially purified by successive column chromatographies on diethylaminoethylcellulose, diethylaminoethyl-Sephadex A-25, hydroxylapatite, and Sephadex G-200. This factor was separated from thermostabl direct hemolysin and was heat labile.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference28 articles.

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