Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli-Reference-Cited by-同舟云学术

Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli

Published:2000-07 Issue:7 Volume:66 Page:3113-3116
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Henne Anke¹,Schmitz Ruth A.¹,Bömeke Mechthild²,Gottschalk Gerhard¹²,Daniel Rolf¹

Affiliation:

1. Abteilung Allgemeine Mikrobiologie1 and

2. Göttingen Genomics Laboratory,2 Institut für Mikrobiologie und Genetik der Georg-August-Universität, 37077 Göttingen, Germany

Abstract

ABSTRACT Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.66.7.3113-3116.2000

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