Detection of Mumps Virus RNA by Real-Time One-Step Reverse Transcriptase PCR Using the LightCycler Platform-Reference-Cited by-同舟云学术

Detection of Mumps Virus RNA by Real-Time One-Step Reverse Transcriptase PCR Using the LightCycler Platform

Published:2008-12 Issue:12 Volume:46 Page:4049-4051
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Leblanc Jason J.¹,Pettipas Janice¹,Davidson Ross J.¹²,Tipples Graham A.³,Hiebert Joanne³,Hatchette Todd F.¹²

Affiliation:

1. Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada

2. Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada

3. National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada

Abstract

ABSTRACT A real-time reverse transcriptase PCR (RT-PCR) assay that targeted both the mumps virus F gene and human RNase P in clinical samples was adapted for use with the LightCycler platform. LightCycler RT-PCR is as sensitive as conventional nested RT-PCR and significantly less expensive and labor-intensive, making it ideal for mumps diagnosis during outbreaks.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01446-08

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