Affiliation:
1. Microbiology and Cell Science Department, University of Florida, Gainesville, Florida 32611-0700
Abstract
ABSTRACT
An enzymatic assay was developed to measure tetrahydromethanopterin (H
4
MPT) levels in wild-type and mutant cells of
Methylobacterium extorquens
AM1. H
4
MPT was detectable in wild-type cells but not in strains with a mutation of either the
orf4
or the
dmrA
gene, suggesting a role for these two genes in H
4
MPT biosynthesis. The protein encoded by
orf4
catalyzed the reaction of ribofuranosylaminobenzene 5′-phosphate synthase, the first committed step of H
4
MPT biosynthesis. These results provide the first biochemical evidence for H
4
MPT biosynthesis genes in bacteria.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
14 articles.
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