Construction and characterization of Escherichia coli genetically engineered for bioremediation of Hg(2+)-contaminated environments

Author:

Chen S1,Wilson D B1

Affiliation:

1. Institute for Comparative and Environmental Toxicology, Cornell University, Ithaca, New York 14853, USA.

Abstract

Escherichia coli strains were genetically engineered to express an Hg2+ transport system and metallothionein. Overexpression of a glutathione S-transferase fusion protein of Saccharomyces cerevisiae or pea metallothionein significantly increased the bioaccumulation of Hg2+ transported by MerT and MerP and protected the cells from the accumulated Hg2+. The recombinant strains have excellent properties for bioremediation of Hg(2+)-contaminated environments.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

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5. Chen S.-L. and D. B. Wilson. Genetical engineering of bacteria and their potential for Hg 2~ bioremediation. Biodegradation in press.

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