Affiliation:
1. Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, Michigan 48823
2. U.S. Geological Survey, Michigan Water Science Center, Lansing, Michigan 48911
Abstract
ABSTRACT
Studies of sulfidic springs have provided new insights into microbial metabolism, groundwater biogeochemistry, and geologic processes. We investigated Great Sulphur Spring on the western shore of Lake Erie and evaluated the phylogenetic affiliations of 189 bacterial and 77 archaeal 16S rRNA gene sequences from three habitats: the spring origin (11-m depth), bacterial-algal mats on the spring pond surface, and whitish filamentous materials from the spring drain. Water from the spring origin water was cold, pH 6.3, and anoxic (H
2
, 5.4 nM; CH
4
, 2.70 μM) with concentrations of S
2−
(0.03 mM), SO
4
2−
(14.8 mM), Ca
2+
(15.7 mM), and HCO
3
−
(4.1 mM) similar to those in groundwater from the local aquifer. No archaeal and few bacterial sequences were >95% similar to sequences of cultivated organisms. Bacterial sequences were largely affiliated with sulfur-metabolizing or chemolithotrophic taxa in
Beta
-,
Gamma
-,
Delta
-, and
Epsilonproteobacteria
.
Epsilonproteobacteria
sequences similar to those obtained from other sulfidic environments and a new clade of
Cyanobacteria
sequences were particularly abundant (16% and 40%, respectively) in the spring origin clone library.
Crenarchaeota
sequences associated with archaeal-bacterial consortia in whitish filaments at a German sulfidic spring were detected only in a similar habitat at Great Sulphur Spring. This study expands the geographic distribution of many uncultured
Archaea
and
Bacteria
sequences to the Laurentian Great Lakes, indicates possible roles for epsilonproteobacteria in local aquifer chemistry and karst formation, documents new oscillatorioid
Cyanobacteria
lineages, and shows that uncultured, cold-adapted
Crenarchaeota
sequences may comprise a significant part of the microbial community of some sulfidic environments.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference58 articles.
1. Angert, E. R., D. E. Northup, A.-L. Reysenbach, A. S. Peek, B. M. Goebel, and N. R. Pace. 1998. Molecular phylogenetic analysis of a bacterial community in Sulphur River, Parker Cave, Kentucky. Am. Mineral.83:1583-1592.
2. Biddanda, B. A., D. F. Coleman, T. H. Johengen, S. A. Ruberg, G. A. Meadows, H. W. Van Sumeren, R. R. Rediske, and S. T. Kendall. 2006. Exploration of a submerged sinkhole ecosystem in Lake Huron. Ecosystems9:828-842.
3. Lake Erie and Lake St. Clair handbook 1993
4. Chemical and isotopic composition and gas concentrations of ground water and surface water from selected sites at and near the Idaho National Engineering and Environmental Laboratory Idaho 1994-97 2000
5. Camacho, A., E. Vicente, and M. R. Miravle. 2000. Ecology of a deep-living Oscillatoria (=Planktothrix) population in the sulphide-rich waters of a Spanish karstic lake. Arch. Hydrobiol.148:333-355.
Cited by
26 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献