Affiliation:
1. Laboratory of Virology, Wageningen University, Droevendaalsesteeg 1, 6708 PB Wageningen, Netherlands
2. Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, Hubei Province, Peoples Republic of China
Abstract
ABSTRACT
Five highly conserved
per os
infectivity factors, PIF1, PIF2, PIF3, PIF4, and P74, have been reported to be essential for oral infectivity of baculovirus occlusion-derived virus (ODV) in insect larvae. Three of these proteins, P74, PIF1, and PIF2, were thought to function in virus binding to insect midgut cells. In this paper evidence is provided that PIF1, PIF2, and PIF3 form a stable complex on the surface of ODV particles of the baculovirus
Autographa californica
multinucleocapsid nucleopolyhedrovirus (AcMNPV). The complex could withstand 2% SDS-5% β-mercaptoethanol with heating at 50°C for 5 min. The complex was not formed when any of the genes for PIF1, PIF2, or PIF3 was deleted, while reinsertion of these genes into AcMNPV restored the complex. Coimmunoprecipitation analysis independently confirmed the interactions of the three PIF proteins and revealed in addition that P74 is also associated with this complex. However, deletion of the
p74
gene did not affect formation of the PIF1-PIF2-PIF3 complex. Electron microscopy analysis showed that PIF1 and PIF2 are localized on the surface of the ODV with a scattered distribution. This distribution did not change for PIF1 or PIF2 when the gene for PIF2 or PIF1 protein was deleted. We propose that PIF1, PIF2, PIF3, and P74 form an evolutionarily conserved complex on the ODV surface, which has an essential function in the initial stages of baculovirus oral infection.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference32 articles.
1. Adams, J. R., and J. T. McClintock. 1991. Baculoviridae. Nuclear polyhedrosis viruses. Part 1: nuclear polyhedrosis viruses of insects, p. 88-226. In J. R. Adams and J. R. Bonami (ed.), Atlas of invertebrate viruses. CRC Press, Boca Raton, FL.
2. Bezier, A., M. Annaheim, J. Herbiniere, C. Wetterwald, G. Gyapay, S. Bernard-Samain, P. Wincker, I. Roditi, M. Heller, M. Belghazi, R. Pfister-Wilhem, G. Periquet, C. Dupuy, E. Huguet, A. N. Volkoff, B. Lanzrein, and J. M. Drezen. 2009. Polydnaviruses of braconid wasps derive from an ancestral nudivirus. Science323:926-930.
3. Braunagel, S. C., and M. D. Summers. 2007. Molecular biology of the baculovirus occlusion-derived virus envelope. Curr. Drug Targets8:1084-1095.
4. Campadelli-Fiume, G., M. Amasio, E. Avitabile, A. Cerretani, C. Forghieri, T. Gianni, and L. Menotti. 2007. The multipartite system that mediates entry of herpes simplex virus into the cell. Rev. Med. Virol.17:313-326.
5. Cumming, R. C., N. L. Andon, P. A. Haynes, M. Park, W. H. Fischer, and D. Schubert. 2004. Protein disulfide bond formation in the cytoplasm during oxidative stress. J. Biol. Chem.279:21749-21758.
Cited by
90 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献