Affiliation:
1. Department of Veterinary and Biomedical Sciences, University of Nebraska—Lincoln, Lincoln, Nebraska 68583-0905
Abstract
ABSTRACT
Previously, we identified bovine CD18 as the receptor for leukotoxin secreted by
Mannheimia
(
Pasteurella
)
haemolytica
. In this study, we constructed bovine-murine CD18 chimeras to locate the leukotoxin binding domain on CD18. Leukotoxin specifically lysed transfectants expressing bovine CD18 fragment encompassing amino acids 1 to 291, indicating that leukotoxin binding domain lies within amino acids 1 to 291 of bovine CD18.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference13 articles.
1. Ambagala, T. C., A. P. N. Ambagala, and S. Srikumaran. 1999. The leukotoxin of Pasteurella haemolytica binds to β2 integrins on bovine leukocytes. FEMS Microbiol. Lett.179:161-167.
2. Berggren, K. A., C. S. Baluyut, R. R. Simonson, W. J. Bemrick, and S. K. Maheswaran. 1981. Cytotoxic effects of Pasteurella haemolytica on bovine neutrophils. Am. J. Vet. Res.42:1383-1388.
3. Bowland, S. L., and P. E. Shewen. 2000. Bovine respiratory disease: commercial vaccines currently available in Canada. Can. Vet. J.41:33-48.
4. Bovine CD18 Is Necessary and Sufficient To Mediate
Mannheimia
(
Pasteurella
)
haemolytica
Leukotoxin-Induced Cytolysis
5. Gentry, M. J., and S. Srikumaran. 1991. Neutralizing monoclonal antibodies to Pasteurella haemolytica leukotoxin affinity-purify the toxin from crude culture supernatants. Microb. Pathog.10:411-417.
Cited by
18 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献