Structural Changes During Lysis of a Psychrophilic Marine Bacterium

Abstract

The marine psychrophile, a red, gram-negative motile rod with a single polar flagellum, is stable when suspended in 0.1 m Mg 2+ plus 0.5 m NaCl at 0 C and neutral p H but lyses if the salt composition of the medium is changed, the temperature raised above 20 C, or the p H lowered. Lysis is accompanied by a fall in turbidity, a release of ultraviolet-absorbing substances, and a loss of deoxyribonucleic acid and ribonucleic acid. Ultrastructural changes accompanying lysis were studied. Thin sections of cells fixed while intact showed a triple-layered cell wall and cytoplasmic membrane, each 6.0 to 7.5 nm thick. Mesosomes were also observed. Either Na + or Mg 2+ could maintain wall integrity, whereas Mg 2+ was needed for membrane integrity. In distilled water, lysis was very extensive, and much material was released as wall fragments and as vesicles which probably came from the wall and cytoplasmic membrane. Lysis at 37 C resulted in degradation of the wall and liberation of wall fragments. The cell membrane was rarely observed as a triple-layered structure in such temperature-lysed cells. After lysis at p H 5.0, the cell wall was distorted, and only a suggestion of the cell membrane remained. Replicas showed that this organism had a matted surface which was distorted under different conditions of lysis.