Enhancement of Recombinant Hemoglobin Production in Escherichia coli BL21(DE3) Containing the Plesiomonas shigelloides Heme Transport System-Reference-Cited by-同舟云学术

Enhancement of Recombinant Hemoglobin Production in Escherichia coli BL21(DE3) Containing the Plesiomonas shigelloides Heme Transport System

Published:2008-09-15 Issue:18 Volume:74 Page:5854-5856
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Villarreal D. M.¹,Phillips C. L.¹,Kelley A. M.¹,Villarreal S.¹,Villaloboz A.¹,Hernandez P.¹,Olson J. S.²,Henderson D. P.¹

Affiliation:

1. Department of Biology, University of Texas of the Permian Basin, Odessa, Texas 79762

2. Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005

Abstract

ABSTRACT To produce recombinant hemoglobin in Escherichia coli , sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) grown in medium containing heme.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01291-08

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