Introduction of the Escherichia coli gdhA gene into Rhizobium phaseoli: effect on nitrogen fixation-Reference-Cited by-同舟云学术

Introduction of the Escherichia coli gdhA gene into Rhizobium phaseoli: effect on nitrogen fixation

Published:1988-02 Issue:2 Volume:170 Page:985-988
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bravo A¹,Becerril B¹,Mora J¹

Affiliation:

1. Centro de Investigación sobre Fijación de Nitrógeno, Universidad Nacional Autónoma de México, Cuernavaca, Morelos.

Abstract

Rhizobium phaseoli lacks glutamate dehydrogenase (GDH) and assimilates ammonium by the glutamine synthetase-glutamate synthase pathway. A strain of R. phaseoli harboring the Escherichia coli GDH structural gene (gdhA) was constructed. GDH activity was expressed in R. phaseoli in the free-living state and in symbiosis. Nodules with bacteroids that expressed GDH activity had severe impairment of nitrogen fixation. Also, R. phaseoli cells that lost GDH activity and assimilated ammonium by the glutamine synthetase-glutamate synthase pathway preferentially nodulated Phaseolus vulgaris.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.170.2.985-988.1988

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