Precise Deletion of tagD and Controlled Depletion of Its Product, Glycerol 3-Phosphate Cytidylyltransferase, Leads to Irregular Morphology and Lysis of Bacillus subtilis Grown at Physiological Temperature-Reference-Cited by-同舟云学术

Precise Deletion of tagD and Controlled Depletion of Its Product, Glycerol 3-Phosphate Cytidylyltransferase, Leads to Irregular Morphology and Lysis of Bacillus subtilis Grown at Physiological Temperature

Published:2001-11-15 Issue:22 Volume:183 Page:6688-6693
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Bhavsar Amit P.¹,Beveridge Terry J.²,Brown Eric D.¹

Affiliation:

1. Antimicrobial Research Centre, Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada L8N 3Z5,1 and

2. Department of Microbiology and Canadian Bacterial Disease Network, University of Guelph, Guelph, Ontario, Canada N1G 2W12

Abstract

ABSTRACT Using a previously reported conditional expression system for use in Bacillus subtilis (A. P. Bhavsar, X. Zhao, and E. D. Brown, Appl. Environ. Microbiol. 67:403–410, 2001), we report the first precise deletion of a teichoic acid biosynthesis ( tag ) gene, tagD, in B. subtilis . This teichoic acid mutant showed a lethal phenotype when characterized at a physiological temperature and in a defined genetic background. This tagD mutant was subject to full phenotypic rescue upon expression of the complementing copy of tagD. Depletion of the tagD gene product (glycerol 3-phosphate cytidylyltransferase) via modulated expression of tagD from the amyE locus revealed structural defects centered on shape, septation, and division. Thickening of the wall and ultimately lysis followed these events.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.183.22.6688-6693.2001

Reference25 articles.

1. Archibald A. R. Hancock I. C. Harwood C. R. Cell wall structure synthesis and turnover Bacillus subtilis and other gram-positive bacteria. Biochemistry physiology and molecular genetics. Sonenshein A. L. Hoch J. A. Losick R. 1993 381 410 American Society for Microbiology Washington D.C.

2. Development and Characterization of a Xylose-Dependent System for Expression of Cloned Genes in Bacillus subtilis : Conditional Complementation of a Teichoic Acid Mutant

3. Regulation of the Bacterial Cell Wall: Analysis of a Mutant of Bacillus subtilis Defective in Biosynthesis of Teichoic Acid

4. Mutants of Bacillus subtilis 168 thermosensitive for growth and wall teichoic acid biosynthesis;Briehl M.;J. Gen. Microbiol.,1989

5. Ultrastructure of a Temperature-Sensitive Rod − Mutant of Bacillus subtilis

Cited by 55 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Serine and threonine activate Ser/Thr kinase gene to increase the density of Lactobacillus bulgaricus sp1.1 under salt stress;LWT;2024-09

2. Mirubactin C rescues the lethal effect of cell wall biosynthesis mutations in Bacillus subtilis;Frontiers in Microbiology;2022-10-13

3. Mircubactin B rescues the lethal effect of cell wall biosynthesis mutations in Bacillus subtilis;2022-04-24

4. A high content microscopy screening identifies new genes involved in cell width control in Bacillus subtilis;2021-06-10

5. A genetic screen to identify factors affected by undecaprenyl phosphate recycling uncovers novel connections to morphogenesis in Escherichia coli;Molecular Microbiology;2020-10-12