groEL Expression in gyrB Mutants of Borrelia burgdorferi-Reference-Cited by-同舟云学术

groEL Expression in gyrB Mutants of Borrelia burgdorferi

Published:2002-11 Issue:21 Volume:184 Page:6069-6072
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Alverson Janet¹²,Samuels D. Scott¹

Affiliation:

1. Division of Biological Sciences, The University of Montana, Missoula, Montana 59812

2. Biological Control and Mass Rearing Research Unit, USDA Agricultural Research Service, Mississippi State, Mississippi 39762

Abstract

ABSTRACT GroEL protein and groEL mRNA transcript were up-regulated in gyrB mutants of Borrelia burgdorferi , a causative agent of Lyme disease. Furthermore, the protein and transcript levels in gyrB mutants were greater than those in experimentally heat-shocked cultures of wild-type B. burgdorferi . Circular DNA in the gyrB mutants was more relaxed than in wild-type cells, although groEL is on the linear chromosome of B. burgdorferi . To our knowledge, this is the first evidence, albeit indirect, for the effect of DNA topology on gene expression from a linear DNA molecule in a bacterium.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.183.21.6069-6072.2002

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