Affiliation:
1. Howard Hughes Medical Institute and Department of Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee
2. The Scripps Research Institute, La Jolla, California
Abstract
ABSTRACT
Schizosaccharomyces
pombe
Dim1p is required for maintaining the steady-state level of
the anaphase-promoting complex or cyclosome (APC/C) component Lid1p and
thus for maintaining the steady-state level and activity of the APC/C.
To gain further insight into Dim1p function, we have investigated the
mechanism whereby Dim1p influences Lid1p levels. We show that
S.
pombe
cells lacking Dim1p or
Saccharomyces cerevisiae
cells lacking its ortholog, Dib1p, are defective in generalized
pre-mRNA splicing in vivo, a result consistent with the identification
of Dim1p as a component of the purified yeast U4/U6.U5 tri-snRNP
complex. Moreover, we find that Dim1p is part of a complex with the
splicing factor Prp1p. However, although Dim1p is required for
efficient splicing of
lid1
+
pre-mRNA, circumventing the necessity for this particular function of
Dim1p is insufficient for restoring normal Lid1p levels. Finally, we
provide evidence that Dim1p also participates in the nuclear export of
lid1
+
mRNA and that it is likely
the combined loss of both of these two Dim1p functions which
compromises Lid1p levels in the absence of proper Dim1p function. These
data indicate that a mechanism acting at the level of mRNA impacts the
functioning of the APC/C, a critical complex in controlling mitotic
progression.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
23 articles.
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