Affiliation:
1. Hospital de Especialidades del Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social Mexico City,
2. Instituto de Diagnóstico y Referencia Epidemiológicos, Secretaría de Salud de México, Mexico City, Mexico
Abstract
ABSTRACT
Neurocysticercosis is a common disease in underdeveloped countries. Its diagnosis is based on clinical, imaging (tomography or magnetic resonance), epidemiological, and laboratory data. Several methods based on the detection of antibodies against cysticerci in cerebrospinal fluid or serum have been tested. Among them, an enzyme-linked immunosorbent assay (ELISA) based on the use of a crude parasite antigen has been used by the laboratory network of cysticercosis in Mexico, which has given support to clinicians for up to 7 years. A
Taenia solium
-specific glycoprotein-based electroimmunotransfer blot (EITB) assay was reported to be highly sensitive and specific for this purpose. In order to compare both techniques, we studied 100 neurocysticercosis patients and 70 neurological noncysticercosis controls and searched for specific antibodies in paired samples of serum and cerebrospinal fluid using both techniques. We found that the EITB assay is more sensitive than the ELISA, especially when serum is being tested. Both techniques are more sensitive in cases with multiple living cysts than in cases with single cysts or calcified lesions. No global differences among cases with parasites located in different parts of the central nervous system were found. In the patients with cysts within the parenchyma, the sensitivity of the EITB assay was higher with serum than with cerebrospinal fluid. The immunodominant bands were found to be the same as those previously reported, i.e., GP-39 to -42, GP-24, and GP-13. Based on these results, we suggest the use of the EITB assay in routine diagnosis of cysticercosis for clinical cases.
Publisher
American Society for Microbiology
Reference16 articles.
1. Correa, D., E. Sarti, E., R. Tapia-Romero, R. Rico, I. Alcántara-Anguiano, A. Salgado, L. Valdez, and A. Flisser. 1999. Antigens and antibodies in sera from human cases of epilepsy or taeniasis from an area of México where Taenia solium cysticercosis is endemic. Ann. Trop. Med. Parasitol.93:69-74.
2. Correa, D., J. V. Proaño, J. C. Allan, A. Meza-Lucas, R. Tapia, I. Alcántara, and O. Mata. 1997. Inmunodiagnóstico de neurocisticercosis mediante la determinación de anticuerpos y antígenos en líquido cefalorraquídeo y suero, p. 279-286. In C. Arriagada, J. Nogales-Gaete, and W. Apt (ed.), Neurocisticercosis. Arrynog Ediciones, Santiago, Chile.
3. Del Brutto, O. H., V. Rajshekhar, A. C. White, Jr., V. C. W. Tsang, T. E. Nash, O. M. Takayanagui, P. M. Schantz, C. A. W. Evans, A. Flisser, D. Correa, D. Botero, J. C. Allan, E. Sarti, A. E. Gonzalez, R. H. Gilman, and H. H. García. 2001. Proposed diagnostic criteria for neurocysticercosis. Neurology57:177-183.
4. Characterization by enzyme-linked immunosorbent assay of the humoral immune response in patients with neurocysticercosis and its application in immunodiagnosis
5. Fleiss J. L. 1983. Statistical methods for rates and proportions 3rd ed. p. 139-162. Wiley New York N.Y.
Cited by
99 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献