High-Throughput Screening and Identification of Potent Broad-Spectrum Inhibitors of Coronaviruses-Reference-Cited by-同舟云学术

High-Throughput Screening and Identification of Potent Broad-Spectrum Inhibitors of Coronaviruses

Published:2019-06-15 Issue:12 Volume:93 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Shen Liang¹,Niu Junwei¹,Wang Chunhua²,Huang Baoying¹,Wang Wenling¹,Zhu Na¹,Deng Yao¹,Wang Huijuan¹,Ye Fei¹,Cen Shan³,Tan Wenjie¹

Affiliation:

1. NHC Key Laboratory of Biosafety, Ministry of Health, National Institute for Viral Disease Control and Prevention, China CDC, Beijing, China

2. National Institutes for Food and Drug Control, Beijing, China

3. Department of Immunology, Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Beijing, China

Abstract

Currently, there is no approved therapy to treat coronavirus infection; therefore, broad-spectrum inhibitors of emerging and endemic CoVs are needed. Based on our high-throughput screening assay using a compound library, we identified seven compounds with broad-spectrum efficacy against the replication of four CoVs in vitro . Additionally, one compound (lycorine) was found to protect BALB/c mice against HCoV-OC43-induced lethality by decreasing viral load in the central nervous system. This inhibitor might offer promising therapeutic possibilities for combatting novel CoV infections in the future.

Funder

Megaproject for Infectious Disease Research of China

National Key Research and Development Program of China

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.00023-19

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