Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved Secretion Mutations-Reference-Cited by-同舟云学术

Functional Expression of Two Unusual Acidic Peroxygenases from Candolleomyces aberdarensis in Yeasts by Adopting Evolved Secretion Mutations

Published:2021-09-10 Issue:19 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Gomez de Santos Patricia¹²,Hoang Manh Dat¹³,Kiebist Jan⁴,Kellner Harald⁵,Ullrich René⁵,Scheibner Katrin⁶,Hofrichter Martin⁵,Liers Christiane⁵,Alcalde Miguel¹^ORCID

Affiliation:

1. Department of Biocatalysis, Institute of Catalysis, CSIC, Madrid, Spain

2. EvoEnzyme S.L., Madrid, Spain

3. Institute of Biochemical Engineering, Technical University of Munich, Garching, Germany

4. JenaBios GmbH, Jena, Germany

5. Department of Bio- and Environmental Sciences, TU Dresden, International Institute Zittau, Zittau, Germany

6. Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany

Abstract

In this work, we incorporated several secretion mutations from an evolved fungal peroxygenase to enhance the production of active and stable forms of two unusual acidic peroxygenases. The tandem-yeast expression system based on S. cerevisiae for directed evolution and P. pastoris for overproduction on an ∼300-mg/liter scale is a versatile tool to generate UPO variants.

Funder

Ministerio de Ciencia, Innovación y Universidades

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00878-21

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