Modified Sialic Acids on Mucus and Erythrocytes Inhibit Influenza A Virus Hemagglutinin and Neuraminidase Functions

Author:

Barnard Karen N.1ORCID,Alford-Lawrence Brynn K.1,Buchholz David W.2,Wasik Brian R.1ORCID,LaClair Justin R.1,Yu Hai3,Honce Rebekah45,Ruhl Stefan6,Pajic Petar6,Daugherity Erin K.7,Chen Xi3,Schultz-Cherry Stacey L.4,Aguilar Hector C.2,Varki Ajit8,Parrish Colin R.1ORCID

Affiliation:

1. Baker Institute for Animal Health, Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA

2. Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA

3. Department of Chemistry, University of California-Davis, Davis, California, USA

4. Department of Infectious Diseases, St. Jude Children’s Research Hospital, Memphis, Tennessee, USA

5. Department of Microbiology, Immunology, and Biochemistry, University of Tennessee Health Science Center, Memphis, Tennessee, USA

6. Department of Oral Biology, University at Buffalo, Buffalo, New York, USA

7. Center for Animal Resources and Education, Cornell University, Ithaca, New York, USA

8. Glycobiology Research and Training Center, University of California, San Diego, California, USA

Abstract

Sialic acids (Sia) are involved in numerous different cellular functions and are receptors for many pathogens. Sia come in chemically modified forms, but we lack a clear understanding of how they alter interactions with microbes. Here, we examine the expression of modified Sia in mouse tissues, on secreted mucus in saliva, and on erythrocytes, including those from IAV host species and animals used in IAV research. These Sia forms varied considerably among different animals, and their inhibitory effects on IAV NA and HA activities and on bacterial sialidases (neuraminidases) suggest a host-variable protective role in secreted mucus.

Funder

HHS | National Institutes of Health

HHS | NIH | National Institute of Allergy and Infectious Diseases

HHS | NIH | National Institute of Dental and Craniofacial Research

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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