Mutational Analysis of the vacA Promoter Provides Insight into Gene Transcription in Helicobacter pylori-Reference-Cited by-同舟云学术

Mutational Analysis of the vacA Promoter Provides Insight into Gene Transcription in Helicobacter pylori

Published:1999-04 Issue:7 Volume:181 Page:2261-2266
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Forsyth Mark H.¹,Cover Timothy L.¹²

Affiliation:

1. Departments of Medicine and Microbiology and Immunology, Vanderbilt University School of Medicine,1 and

2. Veterans Affairs Medical Center,2 Nashville, Tennessee

Abstract

ABSTRACT Analysis of 12 Helicobacter pylori promoters indicates the existence of a consensus −10 hexamer (TAtaaT) but little conservation of −35 sequences. In this study, mutations in either the H. pylori vacA −10 region or the −35 region resulted in decreased vacA transcription and suggested that an extended −10 motif is utilized. Thus, despite the lack of a −35 consensus sequence for H. pylori promoters, the −35 region plays a functional role in vacA transcription.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.181.7.2261-2266.1999

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