Molecular Basis of Macrolide, Triamilide, and Lincosamide Resistance in Pasteurella multocida from Bovine Respiratory Disease

Abstract

ABSTRACTThe mechanism of macrolide-triamilide resistance inPasteurella multocidahas been unknown. During whole-genome sequencing of a multiresistant bovineP. multocidaisolate, three new resistance genes, the rRNA methylase geneerm(42), the macrolide transporter genemsr(E), and the macrolide phosphotransferase genemph(E), were detected. The three genes were PCR amplified, cloned into suitable plasmid vectors, and shown to confer either macrolide-lincosamide resistance [erm(42)] or macrolide-triamilide resistance [msr(E)-mph(E)] in macrolide-susceptibleEscherichia coliandP. multocidahosts.