Overcoming Culture Restriction for SARS-CoV-2 in Human Cells Facilitates the Screening of Compounds Inhibiting Viral Replication-Reference-Cited by-同舟云学术

Overcoming Culture Restriction for SARS-CoV-2 in Human Cells Facilitates the Screening of Compounds Inhibiting Viral Replication

Published:2021-06-17 Issue:7 Volume:65 Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Ramirez Santseharay¹²^ORCID,Fernandez-Antunez Carlota¹²,Galli Andrea¹²^ORCID,Underwood Alexander¹²,Pham Long V.¹²,Ryberg Line A.¹²,Feng Shan¹²,Pedersen Martin S.¹²³,Mikkelsen Lotte S.¹²,Belouzard Sandrine⁴⁵,Dubuisson Jean⁴⁵^ORCID,Sølund Christina¹²⁶,Weis Nina⁶⁷,Gottwein Judith M.¹²,Fahnøe Ulrik¹²,Bukh Jens¹²^ORCID

Affiliation:

1. Copenhagen Hepatitis C Program (CO-HEP), Department of Infectious Diseases, Copenhagen University Hospital, Hvidovre, Copenhagen, Denmark

2. Copenhagen Hepatitis C Program (CO-HEP), Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

3. Department of Clinical Microbiology, Copenhagen University Hospital, Hvidovre, Copenhagen, Denmark

4. University of Lille, CNRS, Inserm, CHU Lille, Lille, France

5. Centre d’Infection et d’Immunité de Lille, Institut Pasteur de Lille, Lille, France

6. Department of Infectious Diseases, Copenhagen University Hospital, Hvidovre, Copenhagen, Denmark

7. Department of Clinical Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

Abstract

Efforts to mitigate the coronavirus disease 2019 (COVID-19) pandemic include the screening of existing antiviral molecules that could be repurposed to treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nuc’s) often show decreased activity in these cells due to inefficient metabolization.

Funder

Candys Foundation

Købmand I Odense Johan og Hanne Weimann Født Seedorffs Legat

Ministry of Science, Innovation and Higher Education | Danish Agency for Science and Higher Education

Novo Nordisk Fonden

Sundhed og Sygdom, Det Frie Forskningsråd

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Pharmacology (medical),Pharmacology

Link

https://journals.asm.org/doi/pdf/10.1128/AAC.00097-21