A Quantitative Metagenomic Sequencing Approach for High-Throughput Gene Quantification and Demonstration with Antibiotic Resistance Genes-Reference-Cited by-同舟云学术

A Quantitative Metagenomic Sequencing Approach for High-Throughput Gene Quantification and Demonstration with Antibiotic Resistance Genes

Published:2021-07-27 Issue:16 Volume:87 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Li Bo¹^ORCID,Li Xu²^ORCID,Yan Tao¹^ORCID

Affiliation:

1. Department of Civil and Environmental Engineering, University of Hawaii at Manoa, Honolulu, Hawaii, USA

2. Department of Civil and Environmental Engineering, University of Nebraska-Lincoln, Lincoln, Nebraska, USA

Abstract

Monitoring and comprehensive assessment of microbial risks in the environment require high-throughput gene quantification. The quantitative metagenomic NGS (qmNGS) approach developed in this study incorporated numerous xenobiotic and synthetic DNA internal standard fragments into metagenomic NGS workflow, which are used to determine a new parameter called sequencing yield that relates sequence base reads to absolute concentration of target genes in the environmental samples.

Funder

U.S. Department of Agriculture

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.00871-21

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