Locations of the hydrogenases of Methanobacterium formicicum after subcellular fractionation of cell extract-Reference-Cited by-同舟云学术

Locations of the hydrogenases of Methanobacterium formicicum after subcellular fractionation of cell extract

Published:1987-08 Issue:8 Volume:169 Page:3823-3825
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Baron S F,Brown D P,Ferry J G

Abstract

The F420 hydrogenase of Methanobacterium formicicum was associated with membranes isolated by sucrose density gradient ultracentrifugation of cell extract. The methyl viologen hydrogenase was present in the soluble fractions. Column chromatography with phenyl-Sepharose CL-4B revealed that the F420 hydrogenase was strongly hydrophobic, suggesting that it associates with isolated membranes through hydrophobic interactions.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.169.8.3823-3825.1987

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